In 2022, there were an estimated 58,224 severe antibiotic-resistant infections and 2,202 associated deaths in England. For more data, see the English surveillance programme for antimicrobial utilisation and resistance report 2022 to 2023. Surveillance of HCAIs and AMU is essential to inform infection prevention control and antimicrobial stewardship interventions. National point prevalence surveys (PPSs) for HCAIs and/or AMU have been conducted in England for the past 40 years. The 2023 HCAI and AMU PPS gathered data across acute, community and mental health NHS Trusts and independent acute-care hospitals in England. Data collection for the 2023 National Point Prevalence Survey (PPS) on healthcare associated infections (HCAI), antimicrobial use (AMU) and antimicrobial stewardship (AMS) in England ended on 7 December 2023. Colleagues from 124 trusts and independent-sector organisations participated – 78 NHS acute trusts, 30 NHS community or mental health trusts and 16 independent sector organisations – and collected data on more than 56,000 patients.